Journal: Aging (Albany NY)

Article Title: Comprehensive analysis of differentially expressed profiles of Alzheimer’s disease associated circular RNAs in an Alzheimer’s disease mouse model

doi: 10.18632/aging.101387

Figure Lengend Snippet: The hierarchical cluster, scatter plot and volcano plot of differential expression of circRNAs in 10-month-old SAMP8 versus age-matched SAMR1 and 10-month-old SAMP8 versus 5-month-old SAMP8. ( A ) Hierarchical cluster of differentially expressed circRNAs. “green” indicates low intensity, “black” indicates medium intensity and “red” indicates strong intensity. ( B ) Scatter plot of circRNA signal values. The values of X and Y axes represents the normalized signal values of the samples (log2 scaled) and the averaged normalized signal values of samples (log2 scaled) respectively. The green lines are fold change lines. The CircRNAs above the top green line and below the bottom green line demonstrates more than 1.5-fold change of circRNAs between the two compared samples. ( C ) Volcano plot of differential expression of circRNAs. The vertical lines correspond to 1.5-fold up and down, respectively. The horizontal line represents a P -value of 0.05, and the red point in the plot represents the differentially expressed circRNAs with statistical significance.

Article Snippet: Three hippocampal tissues of SAMP8 and SAMR1 were used for microarray assay to determine differentially expressed circRNAs using the circRNAs chip (Arraystar mouse circRNAs chip, AraryStar) containing 14,236 probes specific for mouse circular RNAs splicing sites.

Techniques: Quantitative Proteomics

Journal: Aging (Albany NY)

Article Title: Comprehensive analysis of differentially expressed profiles of Alzheimer’s disease associated circular RNAs in an Alzheimer’s disease mouse model

doi: 10.18632/aging.101387

Figure Lengend Snippet: The expression levels of candidate circRNAs for validation by real-time qPCR in 15 10-month-old SAMR1 and SAMP8 hippocampal tissues. Statistically differences were calculated by t -test using SPSS 13.0 software. * P <0.05, ** P <0.01 versus SAMP8 group.

Article Snippet: Three hippocampal tissues of SAMP8 and SAMR1 were used for microarray assay to determine differentially expressed circRNAs using the circRNAs chip (Arraystar mouse circRNAs chip, AraryStar) containing 14,236 probes specific for mouse circular RNAs splicing sites.

Techniques: Expressing, Biomarker Discovery, Software

Journal: Aging (Albany NY)

Article Title: Comprehensive analysis of differentially expressed profiles of Alzheimer’s disease associated circular RNAs in an Alzheimer’s disease mouse model

doi: 10.18632/aging.101387

Figure Lengend Snippet: The expression levels of candidate circRNAs for validation by real-time qPCR in 15 10-month-old SAMP8 and 5-month-old SAMP8 hippocampal tissues. Statistically differences were calculated by t -test using SPSS 13.0 software. ** P <0.01 versus 5-month-old SAMP8 group.

Article Snippet: Three hippocampal tissues of SAMP8 and SAMR1 were used for microarray assay to determine differentially expressed circRNAs using the circRNAs chip (Arraystar mouse circRNAs chip, AraryStar) containing 14,236 probes specific for mouse circular RNAs splicing sites.

Techniques: Expressing, Biomarker Discovery, Software

Journal: Translational Psychiatry

Article Title: Cocaine induces differential circular RNA expression in striatum

doi: 10.1038/s41398-019-0527-1

Figure Lengend Snippet: a The Scatter-Plot is used to analyze the variation of circRNA expression between samples or groups. The values of X- and Y -axis in the Scatter-Plot are normalized (log2 scaled). The green lines represent fold change lines. CircRNAs above the top green line and below the bottom green line indicate more than 2.0 fold change of circRNAs between cocaine group and saline group. b Volcano plot indicates the fold change (two-fold up and down), respectively [Log2 (fold change), x -axis] and p = 0.05 [Log10 ( p value), y -axis] for circRNAs. The red point in the plot represents the differentially expressed circRNA with statistical significance. c Considering the fold change and p -values, 18 circRNAs were up-regulated and 72 were down-regulated. d The distribution of differentially expressed circRNAs in chromosomes

Article Snippet: Limited circRNA probes may attribute to this phenomenon because Arraystar Mouse circRNA Array (6 × 7K, Arraystar) had no circHomer1 probe.

Techniques: Expressing

Journal: Translational Psychiatry

Article Title: Cocaine induces differential circular RNA expression in striatum

doi: 10.1038/s41398-019-0527-1

Figure Lengend Snippet: a The network consists of down-regulated circRNAs (blue nodes) and their target miRNAs (orange nodes). b The network contains up-regulated circRNAs (red nodes) and their target miRNAs (green nodes). c A magnified network of mmu_circRNA_002381, mmu_circRNA_013587, mmu_circRNA_003834 and mmu_circRNA_002520 and their target miRNAs is presented. d The detailed annotation for mmu-miR-138-5p/mmu_circRNA_002381 interaction

Article Snippet: Limited circRNA probes may attribute to this phenomenon because Arraystar Mouse circRNA Array (6 × 7K, Arraystar) had no circHomer1 probe.

Techniques:

Journal: Translational Psychiatry

Article Title: Cocaine induces differential circular RNA expression in striatum

doi: 10.1038/s41398-019-0527-1

Figure Lengend Snippet: a Cocaine self-administration induces mmu_circRNA_002381 and mmu_circRNA_003834 up-regulation, while mmu_circRNA_002520 was down-regulated. b Cocaine hyperlocomotor behavior induces the same modification as those in mmu_circRNA_002381, mmu_circRNA_002520 and mmu_circRNA_003834. c The expression level of mmu_circRNA_002381 was detected after si-mmu_circRNA_002381 treatment in N2a cells. d , e The mRNA and protein expressions of neuroplasticity genes, such as limk1, bdnf, sirt1 , and creb1 were detected following knockdown of mmu_circRNA_002381 using si-mmu_circRNA_002381. f Detailed annotation for mmu-miR-138-5p/mmu_circRNA_003834 interaction is displayed. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Limited circRNA probes may attribute to this phenomenon because Arraystar Mouse circRNA Array (6 × 7K, Arraystar) had no circHomer1 probe.

Techniques: Modification, Expressing

Journal: Cell Death & Disease

Article Title: Angiotensin II inhibits apoptosis of mouse aortic smooth muscle cells through regulating the circNRG-1/miR-193b-5p/NRG-1 axis

doi: 10.1038/s41419-019-1590-5

Figure Lengend Snippet: a Scatter plots were used to evaluate the difference in the expression of circRNAs between Ang II and control groups. The values plotted on X and Y axes are the averaged normalized signal values of each group (log2 scaled). The circRNAs above the top green line and below the bottom green line indicate >1.5-fold change between the two groups. b Hierarchical clustering analysis showed the differentially expressed circRNAs over 2.0-fold change. Red color indicates high expression level, and blue color indicates low expression level. c Divergent and convergent primers were used to verify whether circNRG-1 was a circRNA. Convergent primers were used to detect NRG-1 mRNA. Divergent primers amplified circNRG-1 in cDNA but not gDNA. GAPDH served as linear control and size marker in base pairs. d Sanger sequencing confirmed head-to-tail junction of circNRG-1. e RNA fluorescence in situ hybridization for circNRG-1 was detected. Nuclei were stained with DAPI. Scale bars = 50 μm. f qRT-PCR detected circNRG-1 expression in MASMCs treated with Ang II (10 −7 M) for the different times. Data represent the means ± SEM of three independent experiments. * P < 0.05, *** P < 0.001 vs . Ang II for 0 h

Article Snippet: Circular RNA expression profiling was performed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, USA).

Techniques: Expressing, Control, Amplification, Marker, Sequencing, Fluorescence, In Situ Hybridization, Staining, Quantitative RT-PCR

Journal: Cell Death & Disease

Article Title: Angiotensin II inhibits apoptosis of mouse aortic smooth muscle cells through regulating the circNRG-1/miR-193b-5p/NRG-1 axis

doi: 10.1038/s41419-019-1590-5

Figure Lengend Snippet: The orange, purple and green nodes represent circRNA, miRNA and mRNA respectively. Markers highlighting staining showed circNRG-1-miR-193b-5p-NRG-1 interactions

Article Snippet: Circular RNA expression profiling was performed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, USA).

Techniques: Staining